For analysis, the method of multiplex immunofluorescent analysis and the QuattroPlex device are used

BIOLUMIX reagent kit

A set of reagents for the multiplex detection of IgG antibodies to Toxoplasma gondii, rubella virus, cytomegalovirus and herpes simplex viruses types 1 and 2 in human blood serum by immunofluorescence analysis.
The set of reagents is intended for clinical and epidemiological studies in the conditions of clinical diagnostic laboratories of hospitals, blood service institutions, perinatal centers, antenatal clinics, as well as other medical centers with an obstetric and gynecological focus.


The principle of multiplex analysis

  1. The studied blood serum is mixed with a suspension of polystyrene microspheres encoded with a fluorescent dye in various concentrations and chemically bound to antigens or proteins.
  2. In the case of the presence in the blood serum of IgG antibodies specific to Toxoplasma gondii antigens, rubella virus, CMV and HSV, binding reactions of the antigen immobilized on the surface of the microsphere and the specific antibody occur in the sample.
  3. Next, the suspension of microspheres is washed and a conjugate is added to the washed microspheres, which is an antibody specific for human IgG and labeled with a fluorescent marker (phycoerythrin).
  4. An antigen/specific IgG antibody/conjugate complex is formed on the surface of the microspheres.
  5. The suspension is analyzed using a conventional flow cytometer.

Analysis method

For analysis, the method of flow cytometry is used, while the analysis can be performed on flow cytometers of any manufacturer.
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